Coding

Part:BBa_K1408002:Design

Designed by: Stephen A Rettie   Group: iGEM14_Washington   (2014-10-09)


Gal4-Degron-Vp16


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 870
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 340
    Illegal XhoI site found at 85
    Illegal XhoI site found at 568
    Illegal XhoI site found at 1305
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 487


Design Notes

Xba1, EcoR1 and Spe1 restriction sites were present in the original sequence obtained from the Fields Lab. These are illegal restriction sites according to iGEM rules. The submitted part was synthesized with synonymous substitutions (silent mutations) amplified with primers containing the biobrick prefix and suffix and then digested and ligated into the iGEM vector.

Source

The degron is a Protein domain Saccharomyces cerevisiae MATa2 (a=alpha).

Gal4 is a DNA binding fragment from Saccharomyces cerevisiae. VP16 is a transcriptional activator from herpes simplex virus.

These parts were obtained from the Fields Lab at the University of Washington.


References

Degron from Ben Jester - Fields Lab, UW